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文献和实验8.8,6M Urea,2% Urea,2% DTT,30% glycerol 1 x 10min 375mM Tris-HCl pH8.8,6M Urea,2% Urea,2.5% Iodoacetamide,30% glycerol wash strips with gel running buffer SDS-PAGE Run strips on 12% Acrylamide 18 x 20cm gels 24mA per gel constant,15℃,6 -7 hrs
of gels containing the nonionic detergent Triton X?100, referred to as Triton/acetic acid/urea (TAU) polyacrylamide gels, for analysis of histones. Also included are support protocols detailing several accessory techniques: assembly of gel plates
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.4 - Chromatin Electrophoresis LEVEL II Materials 14 M Urea 6 M NaCl 0.05 M and 0.9 M Acetic acid
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