5′-Deoxy-5′-methylthioadenos 1

Preparation of 2′‐Deoxy‐2′‐Methylseleno‐Modified Phosphoramidites and RNA

of nucleic acids with selenium is a useful approach to facilitate phase determination during three?dimensional structural analysis by X?ray crystallography. This unit describes (1) the synthesis and characterization of 2??deoxy?2??methylseleno (2??Se?methyl

A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes

into CAG-containing genes. (A) Diagrammatic representation of the target sequence including the repeat region (CAG repeat), restriction sites X and Y, where X is the enzyme for the “5′-digestion” and Y is the enzyme for the “3′-digestion” as explained

分子生物学实验诊断技术

清白蛋白），封入袋中37℃，30分钟。100mmol/L Tris-Hcl，100mmol/L NaCl，50mmol/L MgCl2，pH9.5，10ml，1分钟洗一次。取硝基四氮唑兰（NBT）75mg/ml 70％二甲基甲酰胺25μl，4-溴-5-氯-3-吲哚磷酸50mg/ml二甲基甲酰胺20μl（底物）和100mmol/L Tris-HCl，100mmol/L NaCl，50mmol/L MgCl2，pH9.5 6ml混匀，37℃，避光反应三小时，显色。蒸馏水洗膜，晾干。观察结果。