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- Merck
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- 2026年02月12日
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文献和实验实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
he cell suspension at 4,000 RPM in the Sorvall GSA rotor for 10 minutes. Step 5: Resuspend the bacteria pellet on ice in 1/20 volume of ice cold CaCl2 and then add an addional 9/20 volume of CaCl2. Keep this suspension on ice for at least 20 minutes
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