- ￥669
- Merck
- 美国
- 1.12403.0100
- 2026年02月12日
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文献和实验能够非常有效的抑制基因的表达。Figure 1. Silencing Gene Targets by RNase III Derived siRNA Cocktails. A 200 bp dsRNA (15 µg) for each gene of interest was digested with 2.5 U RNase III for 1 hour at 37℃. 1A. RNase III efficiently digests dsRNA . One microgram
CALCIUM CHLORIDE TRANSFORMATION OF BACTERIA WITH PLASMIDS
, but the transformation efficiency of these cells drops rapidly after about a week of storage. Preparation of DNA Q.S. about half of the ligated DNA (usually 25 - 100 ng of vector) to 100 µl with 0.1 M Tris-HCl, pH 7.2. Save the remaining ligated DNA in case
Measurement of Chloride Movement in Neuronal Preparations
. Thampy, K.G. and Barnes, E.M. Jr. 1984. γ‐Aminobutyric acid‐gated chloride channels in cultured cerebral neurons. J. Biol. Chem. 259:1753‐1757.
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