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- 2026年02月12日
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文献和实验In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System
luciferase reporter assay system (DLR, Promega, WI, USA) is designed to control for this technical issue by using a co-transfection approach with two separate reporter proteins that emit at distinct wavelengths: one from firefly (Photinus pyralis
【求助】topflash/foplash的luciferase opening reading fream针对的就是firefly萤光素酶吗？
lope 请教各位大侠： 购买的UPSTATE的TOPFLASH/FOPFLASH质粒，准备做双荧光素酶报告基因检测，看文献上都只是用这个质粒和Renilla的载体共转染细胞，是否这个质粒本身就携带了Firefly萤光素酶呢？我看了说明书，只是说two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse
Promoter Deletion Analysis Using a Dual-Luciferase Reporter System
different substrates to generate quantifiable luminescence. Therefore, the enzymatic activities of firefly and Renilla luciferases can be sequentially measured in a single sample by controlling reaction conditions. Here, we describe a dual-luciferase
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