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文献和实验Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains
The cargo-binding domain of the cytoplasmic dynein complex consists of an intermediate chain, a light-intermediate chain, and three families of light chains. These five subunits form the base of the dynein complex. Variations
Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena
In this chapter, we describe our methods to evaluate the dynein heavy chain genes expressed in a cell. Dynein is the high molecular weight molecular motor that produces directed movement along microtubules. All known dyneins travel
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
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