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文献和实验We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently
淀粉和支链淀粉的未知样品,与碘显色后,只要在选定的波长λ1 ,λ 2 , λ 3 ,λ 4 ,处作 4 次比色,利用直链淀粉和支链淀粉标准曲线即可分别求出样品中两类淀粉的含量。三、仪器、试剂和材料1. 仪器(1) 电子分析天平(2 )索氏脂肪抽提器 1 套(3 )分光光度计 1 台(4 ) pH 计(5 )容量瓶 100ml X 2 , 50ml X 16(6 )吸管 0.5ml X 1, 2ml X 1, 5ml X 12. 试剂( 1) 乙醚或石油醚(沸程 30-60 ℃)(2 )无水
at least 2 mg (up to 4mg) of total RNA in 750 l RNase-free water. In general, inputting more total RNA will yield more polyA RNA. This protocol is most successful with inputs of at least 2 mgs. (Less than 1mg of total RNA input is NOT recommended
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