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文献和实验Preparation of Sonicated Human DNA
Purpose: To break up high molecular weight human placental DNA into fragment sizes of 500 bp or less which can be used as competitor DNA in Southern and in situ hybridizations. This method can also be used for sonicating salmon
Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
complex that biotin forms with streptavidin. In this procedure, a DNA fragment is prepared that contains a high?affinity binding site for the protein of interest, and a molecule of biotinylated nucleotide is then incorporated into one of the ends
Analysis of DNA‐Protein Interactions Using Proteins Synthesized In Vitro from Cloned Genes
‐dC) or other bulk carrier DNA recipeLoading buffer 45% methanol/10% acetic acid
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