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文献和实验Purification of Myosin Light Chain Kinase
)2 - 8ml of 1M stock solution 200 mM NaCl- 23.4g 1 mM DTT* - 308mg 10 μmleupeptin* - 2ml of 5mg/ml stock solution 0.1 mM PMSF* - 3.6ml of 10mg/ml stock solution 1 μg/ml Pepstatin A* - 2ml of 1mg/ml stock solution pH 7.0 9.PMSF 500 mg 10mg/ml 50 ml
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
, calmodulin‐dependent kinase II, CDK5, and Erk1. Mol. Cell. Biol. 16:6486‐6493. Yaffe, M.B., Leparc, G.G., Lai, J., Obata, T., Volinia, S., and Cantley, L.C. 2001. A motif‐based
V294.PART II,Chapter 2 Boyden法体外趋化试验
., and Park, M. (2000)Activation of Cdc42, Rac, PAK, and Rho-kinase in response to hepatocyte growth factor differentially regulates epithelial cell colony spreading and dissociation. Mol. Biol. Cell 11, 1709–1725. 8. Ren, X. D., Kiosses, W. B., Sieg, D
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