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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
I-A/I-E Monoclonal Antibody (M5/114), FITC
- 靶点:
I-A/I-E
- 应用范围:
Flow Cytometry
- 宿主:
Rat
- 适应物种:
Mouse
- 克隆性:
Monoclonal
- 保存条件:
4° C, store in dark, DO NOT FREEZE!
- 形态:
Liquid
- 亚型:
IgG2b
- 免疫原:
Activated C57BL/6 mouse spleen cells
- 规格:
100 ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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