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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20℃
- 保质期:
1year
- 库存:
详询
- 供应商:
杭州臻优品生物科技有限公司
- 规格:
10ug
缓 冲 液:0.01mol/L的PBS、pH:7.2、0.2%Procline-300。
表达宿主:E.coli
内毒素:经LAL检测验证,小于0.1ng/ul(0.1EU/ug)
纯化方法:亲和纯化。
应用范围:用于细胞培养的刺激剂;用于ELISA、CLIA、WB、IHC等免疫检测。
产品纯度:不低于总蛋白的90%。
储存方法:冻干蛋白存放在-20℃保存1年;复溶后蛋白溶液在无菌条件下-20℃至-80℃保存3个月;复溶后蛋白溶液在2-8℃保存2-7天。
注意事项:避免反复冻融。
有效期限:请您在管体注明有效期内使用。
使用建议:本产品可以用于细胞水平、动物水平、蛋白水平的免疫学反应相关实验。
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文献和实验Purification of acidic phosphatase from mustard seedlings
phosphatases are widely distributed in many organisms, including plants. They work optimally at ~ pH 5 without additional cofactors. The enzymes are classified as E.C. 3.1.3.2. In this experiment, we will extract an acidic phosphatase from seedlings
Purification of acidic phosphatase from mustard seedlings
1 ml aliquot to an Eppendorf microfuge tube, and label as SN1 and leave on ice. This will be used for subsequent protein and activity determination. Later freeze at -20 °C. Resuspend the pellet which contains the cell debris and nuclei in 10 ml water
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
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