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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Ally 2,4,6-tri-O-benzoyl-alpha-D-galactopyranoside
- 供应商:
天津本生生物
- CAS号:
1367797-62-3
- 规格:
1g
烯丙基2,4,6-三-O-苯甲酰基-α-D-吡喃半乳糖苷Ally 2,4,6-tri-O-benzoyl-alpha-D-galactopyranoside
产品编号:A10926
规格:A10926-1g
Cas号:1367797-62-3
规格:1g
中文名称:烯丙基2,4,6-三-O-苯甲酰基-α-D-吡喃半乳糖苷
英文名称:Ally 2,4,6-tri-O-benzoyl-alpha-D-galactopyranoside
纯度:98%
烯丙基2,4,6-三-O-苯甲酰基-α-D-吡喃半乳糖苷仅用于科研实验!
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文献和实验邻硝基苯-β-吡喃半乳糖苷 O-nitrophenyl-β-D-galactopyranoside
缩写为 ONPG。是广泛利用于β -半乳糖苷酶活力测定的底物。水解后可游离出邻硝基酚,由于邻硝基酚在碱性条件下呈黄色( 420毫微米光吸收),所以容易进行比色定量。另外利用这种性质,还可用于分离各种代谢调节突变株。
Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
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