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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
information Moreover, as this procedure is quite complex, the use of commercial kits such as ApoTagTM (Oncor, Gaithersburg, MD, USA) and "In situ cell death detection Kit" (Boeringer-Mannheim, Germany), is recommended. Utilizing
protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085): 1. Incubation of cells with Annexin V-FITC 1) Induce apoptosis by desired method. 2) Collect 1-5 x 105 cells by centrifugation. 3) Resuspend cells
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
precipitate is superior to that of X-gal and thus PLAP is the enzyme of choice when electron microscopy is required. There are endogenous alkaline phosphatase activities that may lead to difficulties in detection of transduced PLAP (McComb et al., 1979
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