DNA Polymerase I (E.coli)

Maxiprep of plasmid DNA from E.coli protocol

  Solutions/reagents: LB broth + selective marker 50% sterile glycerol TEG (25mM Tris-Cl, 10mM EDTA, 50mM dextrose) 20 mg/ml lysozyme 10% SDS 4M NaOH autoclaved water Solution 3 (3M potassium-acetate, 2M acetic acid

DNA Polymerase Profiling

We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template

The Polymerase Chain Reaction (PCR)

. Did He Really Invent PCR?　　• The basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khorana in 1971:– Kleppe et al. (1971) J. Mol. Biol. 56, 341-346.　　• Progress was limited by primer synthesis and polymerase