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文献和实验E.Z.N.A. Cycle-Pure Kit Spin Protocol
of the sample at 260nm and then at 280nm. The DNA concentration is calculated as follows: 260 DNA concentration = A x 50 x (Dilution Factor) ug/ml Fragments greater than 500bp in length can routinely be purified at > 80% yield. 260 Bands ranging
【资源】Ribozymes and siRNA protocols 2e (Humana Press)
/Ribozyme Transgenes 15. Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases 16. Helicase-Attached Novel Hybrid Ribozymes 17. Functional Gene Discovery Using Hybrid Ribozyme Libraries 18. Maxizyme Technology 19
Healthcare Life Sciences #17 - 1440 - 02) RPMI 1640 培养基(Invitrogen) 胎牛血清(FBS)(GIBCO) 双抗 P/S(Penicillin/ Streptomycin) (GIBCO) 二甲亚砜(DMSO)(SIGMA) 预先装好异丙醇的冻存盒 2. 方法/步骤 配制所需的溶液: a. 细胞培养基:RPMI 1640 + 10% FBS+ 1% P/S; b. 细胞冻存液:FBS 中加入 10%DMSO; (1)在离心管中加入淋巴
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