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Sequencing Reaction CleanupMontage SEQ kits provide efficient, centrifugation-free sequencing reaction cleanup in 96 and 384-well formats. The unique size-exclusion separation technology eliminates the need for costly gel filtration resins or bind-wash-elute columns. In addition, the kits are optimized for use with BigDye v 3.0 and DYEnamic ET Terminator sequencing chemistries. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/LSKSBW500
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PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. x. Place 50 µL of the biotin-avidin-HRP mix on the preparation and cover with a coverslip. Incubate the slides for 40 min at room temperature in a humid chamber. xi. Wash slides in wash solution 1 for 15 min
Steady State ATPase Assays Coupled Enzyme System
340 at 10 second intervals for 300 seconds. A rate factor of 6.220 x 106 cm2 at 340 nm = molar extinction coefficient of NADH can be entered into the data collection program. 4. Wash and invert 4 cuvettes to dry
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