GE 17145501 PRECISION COLUMN H

ChIP-Chip E. coli

the DNA: BioPrime kit Invitrogen (18094-011)[1] Cy3-dCTP GE Healthcare (PA53021)[2] Cy5-dCTP GE Healthcare (PA55021) [3] Deoxynucleotides (2mM dATP, 2mM dGTP, 2mM dTTP, 0.5mM dCTP mix). Prepared as follows: 4μl 100mM

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

guideline is presented, based on the measured sedimentation coefficient and a calculated maximum S , to estimate if a protein is globular or elongated. It is recalled that a gel filtration column fractionates proteins on the basis of their Stokes radius

Single-molecule analysis of DNA replication by molecular combing

. Add an equal volume of molten LMP agarose (42°C) and prepare agarose plugs using a plug mold (GE Amersham). Each plug (90 µl) should contain 5.107 cells or approximately 850 ng of genomic DNA. Let agarose plugs solidify for 30 minutes at 4°C