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文献和实验Detailed understanding of chemokine action ultimately requires molecular cloning of chemokine receptor genes and cDNAs. Both forward and reverse genetic methods have been used for this purpose (1 ). Forward methods, which involve primary
Epigenetic Regulation of Chemokine/Chemokine Receptor Expression
for several members of the CXC (ELR+ ) chemokine family. In addition we describe a chromatin immunoprecipitation assay to determine the association between chromatin transcription markers and DNA following pretreatment of cell cultures with an HDi, Trichostatin A (TSA
Chemokine Receptor Antagonist Development
This chapter describes assays that focus on the characterization of compounds identified in high-�throughput screening campaigns, and the subsequent medicinal chemistry programs. They cover methods to determine potency
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