anti-DEAD-box protein 6

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN

  STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN Indirect Method Materials 1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802), prepare 2 mg/ml stock in PBS solution A, clarify 25,000 rpm for 20 min in a 42

Identification of Protein Interactions by Far Western Analysis

far western blotting, a method of identifying protein?protein interactions. In a far western blot, one protein of interest is immobilized on a solid support membrane, then probed with a non?antibody protein. As described, far western blots can be used

Single Molecule FRET Characterization of Large Ribozyme Folding

studies on the folding and dynamics of a large ribozyme in the presence of metal ions (i.e., Mg2+ and Ca2+ ) and/or the DEAD-box protein Mss116 as an example, we here describe step-by-step procedures to perform experiments. smFRET allows the investigation