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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
inactivated Salmonella enterica
- 形态:
Liquid
- 保存条件:
4°C up to one month or in aliquots at -20°C for lo
- 克隆性:
Polyclonal
- 适应物种:
Salmonella enterica
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
To be determined by end user.
- 靶点:
Salmonella is a genus of rod-shaped, Gram-negative, non-spore forming, predominantly motile enterobacteria with diameters around 0.7 to 1.5 µm, lengths from 2 to 5 µm, and flagella which project in all directions. Salmonella are closely related to the Esc
- 抗体名:
Salmonella, Serum (5 ml)
- 规格:
5 ml
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文献和实验Isolation of complexes from serum
Complexes can be isolated from serum by centrifugation on either a sucrose or Percoll gradient. The sucrose gradient is the easier of the two to set up. Sucrose gradient: Place 5 ml of 50% sucrose
ml bottles) for 15 min. 4. Pull off serum by aspiration (peelet is soft and will not stick to bottle). 5. Filter serum successively through 1.2 µm, .85µm and .65 µm filters using 142 mm filter holder. 6. Adjust pH to 7.0 using 1N NaOH. 7. Heat
ELISA Procedure for Measuring Serum Antibody Titer
assays are the most appropriate for measuring the titer of the antisera you have generated. ELISA Procedure for Measuring Serum Antibody Titer In this type of ELISA (Enzyme-Linked Immunosorbant Assay), the antigen (peptide or protein) is bound
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