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文献和实验acetone for 10min, washed three times with PBS, acted upon by 0.25%～0.5% Triton X-100 for 10min, and washed Thrice with PBS. Bcl-2 and Bax protein in the BEL-7402 cells were detected with SABC method. The cell specimen s were incubated with 0.3% hydrogen
holybible1263 请问体外做hepG-2,体内做H22，有可比性吗？ fuxiao29 我个人认为体外做hepG-2实验,体内也应该用同一种细胞系做，这样才有可比性。 holybible1263 谢谢，不过HEPG-2没有办法做体内呀，体内要用什么呢？ fuxiao29 可以做体内实验呀，你可以将你用的质粒转染到HEPG-2细胞系里，构建稳定
. None of the cell lines and tumours showed structural changes in the Rb gene, while 6 cell lines and 2 tumours had mutation or deletion in exons 5 to 8 of p53. Mutations include an AGG --> AGT (Arg --> Ser) transversion at codon 249 in PLC/PRF/5 and Mahlavu, an AAT
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