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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH 8.0 by gently pipetting up and down with a 10 ml pipette do not vortex. Cells should be resuspended
的伏小时数的设置及说明 胶条长度 7cm 11cm 13cm 17cm 18cm 24cm 上样体积 125ul 185ul 250ul 300ul 350ul 450ul 银染上样量范围 pH3-10L (ug) 5-100 15-200
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH
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