CM0933B KF STREPTOCOCCUS AGAR

Enterococci

. faecalis and S.faecium . The medium employed for enumeration of coliforms in foods is KF agar.

A novel method of growing fungi for DNA extraction

agar with cold water significantly improved yields, but DNA also was isolated from unwashed mycelium. The resulting DNA performed normally in the ITS PCR amplification and subsequent restriction digests (Figure 1). Figure 1. Miniprep DNA (b-d

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

Figure 2). Red/ET competent bacteria carrying the mLhcgr -BAC were transformed with the I-SceI-CS PCR product and plated on LB agar plates supplemented with Km/Cm. Positive clones were screened by PCR (primers mLhcgrP-F/mLhcgr3-R) and further selected