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文献和实验of acetic acid Make 50% Methanol: 200 ml of water + 200 ml of methanol. Optional � for extra fixation/crosslinking add 240 µl of 50% glutaraldehyde to the 50% methanol (makes solution 0.03% glutaraldehyde). Soak gel in 7% acetic acid
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. 0.5% glutaraldehyde Make from a 25.0% stock immediately before use. You can buy a 25% solution (Sigma) and freeze-thaw it many times. 4.0% paraformaldyde 4 g paraformaldehyde 2 mM MgCl2 (0.2 ml of a 1 M stock) 1.25 mM EGTA (0.25 ml of a .5M
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
In contrast with other methods using gonads affixed to slides, the various manipulations described below (gonad dissections, hybridizations, and washes) are all done in solution, either in a glass dishes (dissections) or in small glass
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