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文献和实验a cell suspension in HBSSTransfer into Eppendorf tube:0.5 ml of 0.4% Trypan blue solution0.3 ml of HBSS0.2 ml of cell suspension in HBSS (= dilution 1 : 5)Allow to stand for 5 to 15 minutesNote: after prolonged incubation, viable cells start
LIVE/DEAD® Violet Viability/Vitality Kit
viability and cell vitality depends on these physical and biochemical properties of the cells. As cells die, variations in fluorescence will be observed. 实验试剂 Table 1. Contents and storage information
Trypan blue viability test Outline: Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note : Trypan blue has a greater affinity for serum proteins than for cellular protein
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