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文献和实验Staining Methods for cell death
Staining Methods for cell death Z. Xia 10/2/95 The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead cells
where C = cells per milliliter Ñ = average of cells counted 104 = volume conversion factor for 1 mm2 Total Yield = C x V where V = total vlaue of cells (ml) Example: Count
Infection with retroviruses It is well established with avian retroviruses that cells are most efficiently infected just after they are trypsinized. Trypsinization does two things. First, it apparently exposes the receptor
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