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文献和实验GSP融合蛋白的准备 GST Fusion Protein Prep
GST Fusion Protein Prep Italics indicate optional steps especially useful for the analysis of untested proteins. GROWTH AND HARVESTING OF BACTERIA Add 100 ul ampicillin to 100 ml LB. Inoculate and grow overnight.
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
- 2% of aliquot of an eluate fraction (step 26): 10 µl The GST moiety adds ~26 kD molecular mass. If protein degradation is occurring, the MW of the majority recovered species may be significantly less than the predicted MW; run the gel
Synthetic microRNA Targeting Glioma-associated Antigen-1 Protein
The transcription factor glioma-associated antigen-1 (Gli-1) mediates activation of the sonic hedgehog (Shh) pathway, a process that precedes the transformation of tissue stem cells into cancerous stem cells and that is involved in early
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