微生物冻干保护剂

使用方法：

1. Simple Culture Preservation:

Add an appropriate volume Lyophilized Buffer to a sterile vial(e.g., 100-500ul). Inoculate the buffer with 10ul of actively growing bacteria. All procedures must be performed aseptically.

2.Preservation of Large Number of Cells:

Pellet cells from an actively growing culture by centrifugation. Decant the medium and resuspend the cell in an equal volume of Lyophilized Buffer. Transfer the cell solution to a sterile vial.

The freeze drying parameters must be determined for each freeze dryer. However the buffer are

optimized to freeze drying on the following cycle.

Freezing:30 minutes from ambient to -40℃, hold for 1 hr.

Primary Drying: Increase temperature to -10℃, hold for 16 hours(large volumes

may require longer reying times).

Secondary Drying:Increase temperature to 20℃, hold for 2 hr.