Add an appropriate volume Lyophilized Buffer to a sterile vial(e.g., 100-500ul). Inoculate the buffer with 10ul of actively growing bacteria. All procedures must be performed aseptically.
2.Preservation of Large Number of Cells:
Pellet cells from an actively growing culture by centrifugation. Decant the medium and resuspend the cell in an equal volume of Lyophilized Buffer. Transfer the cell solution to a sterile vial.
The freeze drying parameters must be determined for each freeze dryer. However the buffer are
optimized to freeze drying on the following cycle.
Freezing:30 minutes from ambient to -40℃, hold for 1 hr.
Primary Drying:Increase temperature to -10℃, hold for 16 hours(large volumes
may require longer reying times).
Secondary Drying:Increase temperature to 20℃, hold for 2 hr.