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文献和实验1. Establishment of fibroblast cultures from skin explants 1) 4 mm punch biopsies acquired from human forearm are placed in MEM supplemented 100 units / ml penicillin, 100 μg / ml streptomycin, and 0.25 μg / ml Fungizone.
of the sperm. We recommend that frozen sperm be tested for successful IVF before a line is discontinued. 6. If the line was derived from an ES cell clone, it can be re-injected to generate a new set of germline chimeras. 7. If the line is available
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
with the support of OP9-DL1 cells, a bone-marrow-derived stromal cell line that ectopically expresses the Notch ligand, Delta-like 1 (Dll1). This approach provides a simple, versatile, and efficient culture system that allows for the commitment, differentiation
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