DNA结合蛋白2抗体

英文名称SATB2中文名称DNA结合蛋白2抗体别    名DNA binding protein SATB2; DNA-binding protein SATB2; FLJ21474; FLJ32076; KIAA1034; MGC119474; MGC119477; SATB family member 2; SATB homeobox 2; SATB2; SATB2_HUMAN; Special AT rich sequence binding protein 2; Special AT-rich sequence-binding protein 2.  研究领域发育生物学  神经生物学  信号转导  结合蛋白  抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat,  (predicted: Cow, Horse, Sheep, )产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.分 子 量83kDa细胞定位细胞核 性    状Liquid浓    度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human SATB2:451-485/733 亚    型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍SATB2 is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.

Function:
Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

Subunit:
Interacts with ATF4 and RUNX2; resulting in enhanced DNA binding and transactivation by these transcription factors (By similarity). Interacts with PIAS1.

Subcellular Location:
Nucleus matrix.

Tissue Specificity:
High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

Post-translational modifications:
Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.

DISEASE:
Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).

Similarity:
Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.

SWISS:
Q9UPW6

Gene ID:
23314

Database links:

Entrez Gene: 23314 Human

Entrez Gene: 212712 Mouse

Entrez Gene: 501145 Rat

Omim: 608148 Human

SwissProt: Q3ZB87 Human

SwissProt: Q4V763 Human

SwissProt: Q9UPW6 Human

SwissProt: Q546B3 Mouse

SwissProt: Q8VI24 Mouse

SwissProt: D3ZJ19 Rat

Unigene: 516617 Human

Unigene: 145599 Mouse

Unigene: 216103 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.