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文献和实验Chondrogenic Differentiation of hESC in Micromass Culture
Human adult cartilage has limited capacity for self-renewal. Accordingly, repair of cartilage tissue damaged as a result of acute traumatic injury or via chronic wear or degenerative disease, such as arthritis, is a major clinical problem
Culture of Human Embryonic Stem Cells (hESC)
at the same density. Immediately before plating hESC, MEFs are rinsed once or twice with PBS. hESC are plated onto MEFs as small clumps in 2.5ml per well of hESC medium containing 4ng/ml bFGF (R&D Systems Cat #233-FB). Cells are fed every day until ready
hESC Engineering by Integrase-Mediated Chromosomal Targeting
to deliver a chromosomal target for a second integrase, R4. The engineered hESC line can be adapted for complex element assembly using Multisite Gateway technology. Retargeted clones show sustained expression and appropriate regulation of the transgenes
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