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Epredia艾普迪 CryoStar NX50 冷冻切片机
1.大型开放式冷冻室具有明亮的可调节 LED 照明、 18 个组织工作站和一个快速冷冻站点,可快速高效地进行样本制备
2.可选的 Cold-D 消毒系统可提供高效灭菌,可使冷冻切片机在低温状态时在60分钟内显著降低微生物感染的风险
3.轻巧的大手轮,减少了重复运动的不适感。电动切片有助于减轻用户疲劳并避免重复性运动伤害
4.符合身体轮廓的弧形设计使用户身体更靠近冷冻室,保持舒适的姿势
5.内置马达驱动的高度调节(82-112 厘米)功能,用户只需按一下按钮即可以选择站位或坐位舒适地工作(NX50 型号的可选配置)
6.内置触摸屏易于操作使用
型号:
CryoStar NX50 OP, 220-230V 957220LC
CryoStar NX50 OPD, 220-230V 957140LC
CryoStar NX50 OVP, 220-230V 957200LC
CryoStar NX50 HOP, 220-230V 957210LC
CryoStar NX50 HOPD, 220-230V 957150LC
CryoStar NX50 OVPD, 220-230V 957160LC
CryoStar NX50 HOVP, 220-230V 957170LC
CryoStar NX50 HOVPD, 220-230V 957240LC
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文献和实验[1] Wang F, Ma S, Chen P, Han Y, Liu Z, Wang X, Sun C, Yu Z. Imaging the metabolic reprograming of fatty acid synthesis pathway enables new diagnostic and therapeutic opportunity for breast cancer. Cancer Cell Int. 2023 Apr 29;23(1):83. doi: 10.1186/s12935-023-02908-8. PMID: 37120513; PMCID: PMC10149015.
[2]Spera I, Cousin N, Ries M, Kedracka A, Castillo A, Aleandri S, Vladymyrov M, Mapunda JA, Engelhardt B, Luciani P, Detmar M, Proulx ST. Open pathways for cerebrospinal fluid outflow at the cribriform plate along the olfactory nerves. EBioMedicine. 2023 May;91:104558. doi: 10.1016/j.ebiom.2023.104558. Epub 2023 Apr 10. PMID: 37043871; PMCID: PMC10119713.
[3]Zhang J, Feider CL, Nagi C, Yu W, Carter SA, Suliburk J, Cao HST, Eberlin LS. Detection of Metastatic Breast and Thyroid Cancer in Lymph Nodes by Desorption Electrospray Ionization Mass Spectrometry Imaging. J Am Soc Mass Spectrom. 2017 Jun;28(6):1166-1174. doi: 10.1007/s13361-016-1570-2. Epub 2017 Feb 28. PMID: 28247296; PMCID: PMC5750372.
[4]Zhang L, Li J, Lin A. Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice. STAR Protoc. 2021 Oct 25;2(4):100915. doi: 10.1016/j.xpro.2021.100915. PMID: 34755118; PMCID: PMC8556527.
[5]Gijs M, Penner G, Blackler GB, Impens NR, Baatout S, Luxen A, Aerts AM. Improved Aptamers for the Diagnosis and Potential Treatment of HER2-Positive Cancer. Pharmaceuticals (Basel). 2016 May 19;9(2):29. doi: 10.3390/ph9020029. PMID: 27213406; PMCID: PMC4932547.
实验试剂 1. 样品包埋剂选用Leica CM1900 冷冻切片机配套包埋剂(OCT-compound) 2. 结晶紫,组织染色选用常规染色剂 3. 二甲苯(进行组织切片的脱水和透明处理) 4. 香柏油(油镜观察用) 实验设备 1. Leica CM1900 冷冻切片机(Leica Microsystems Nussloch GmbH, German
Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
growing 2-4 cryovials Procedure: Frozen cells should be thawed quickly. Remove the cryovial from its long term storage container in the -135 degrees C Cryostar, and place immediately in a 37 degrees C water bath for 2 minutes.
水溶解成 1000ml 按下表混合甲、乙两液即得所需PH的磷酸缓冲液 表1:0.2M的磷酸缓冲液的配制(50ml) PH 6.4 6.6 6.8 7.0 7.2 7.4 甲液(ml) 13.3 18.8 24.5 30.5 36.0 40.5 乙液(ml) 36.7 31.2 25.5 19.5 14.0 9.5 磷酸盐缓冲液对细胞无毒性作用,常用于配制戊二醛固定液,并适于作灌注
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资料下载:
NX50 Mouse Brain Section.pdf 附 (下载 0 次)
NX50 Mouse Tumor Frozen Section.pdf 附 (下载 0 次)
NX50 Human Breast Cancer Section.pdf 附 (下载 0 次)
NX50 Mouse Decalcified Head Section.pdf 附 (下载 0 次)
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