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- Cell Signaling Technology
- USA
- 58214T
- 2026年01月30日
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文献和实验Dynabeads® Co-Immunoprecipitation Kit
Co-Immunoprecipitation Optimization below) from which the desired protein complex is isolated. The Extraction Buffer is made with 5 × IP supplied in the kit. The 5 × IP is supplied as 5 × concentrate and in sufficient quantity to allow for 40 coimmunoprecipitation
of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
(700 to 1000 rpm at 4�C, ~1min). Carefully remove the supernatant that contains unbound, non-specific DNA. Wash the protein A agarose/antibody/histone complex for 3-5 minutes on a rotating platform with 1ml of each of the buffers listed in the order
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