- 询价
- Cell Signaling Technology
- USA
- 76713S
- 2026年01月30日
- IHC-P
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃
- 应用范围：
IHC-P
- 规格：
1Kit
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
. DO NOT DIFFERENTIATE IN ACID ALCOHOL. 26. Mount in an AQUEOUS MOUNTANT. Results --Nuclei Blue --Sites of alkaline phosphatase activity Red Substrate Solution Naphthol-AS-MX phosphatase, free acid (Sigma N 4875) 2 mg N,N-Dimethyl formamide
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
in a light orange-pink precipitate which develops slowly. Adding tetrazolium red or blue gives a purple precipitate similar to XP + NBT. The Vector Red substrate kit (Vector Laboratories) results in staining that is highly variable between experiments
cells. The detection of marker gene products such as β-galactosidase (β-gal), chloramphenicol acetyltransferase (CAT), alkaline phosphatase, or luciferase involves either cell fixation, which kills the cells or antibody-mediated detection, which is time
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