Phospho-NF-κB p65 (Ser536) (7F

3036:

Western Blotting

Phospho-NF-kappaB p65 (Ser536) (7F1) Mouse mAb detects NF-kappaB p65 only when phosphorylated at serine 536. It does not cross-react with the p50 subunit or other related proteins.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65.

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or TNF-alpha-treated (#2169, 20ng/ml), using Phospho-NF-kappaB p65 (Ser536) (7F1) Mouse mAb (upper) or NF-kappaB p65 Antibody #3034 (lower).

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

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• 4764 NF-κB p65 (C22B4) Rabbit mAb
• 3034 NF-κB p65 Antibody
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 3039 Phospho-NF-κB p65 (Ser468) Antibody
• 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
• 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
• 9243 NF-κB Control Cell Extracts
• 9936 NF-κB Pathway Sampler Kit
• 7072 Phototope^® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.