Recombinant Human TFIIB/GTF2B/

Recombinant Human TFIIB/GTF2B/Transcription initiation factor IIB (C293)

 

产品说明(Description)

Recombinant Human Transcription Initiation Factor IIB is produced by our E.coli expression system and the target gene encoding Met1-Leu316 is expressed with a GST tag at the N-terminus. 

Accession #: Q00403

Known as: Transcription Initiation Factor IIB; General Transcription Factor TFIIB; S300-II; GTF2B; TF2B; TFIIB

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, pH 8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than85% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY