Recombinant Human D-amino-acid

Recombinant Human D-amino-acid oxidase/DAO  (CE63)

 

产品说明(Description)

Recombinant Human D-Amino-Acid Oxidase is produced by our E.coli expression system and the target gene encoding Met1-Leu347 is expressed with a 6His tag at the N-terminus. 

Accession #: P14920

Known as: D-Amino-Acid Oxidase; DAAO; DAMOX; DAO

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 20% Glycerol, 1mM DTT, 2mM EDTA, pH8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

1、Discovery and analgesic evaluation of 8-chloro-1,4-dihydropyrido[2,3- b]pyrazine-2,3-dione as a novel potent D-amino acid oxidase inhibitor. Dongsheng Xie. et al. European Journal of Medicinal Chemistry. 2016.

 

 

FOR RESEARCH USE ONLY