Recombinant Human Alkaline Pho

Recombinant Human Alkaline Phosphatase/ALPL  (C554)

 

产品说明(Description)

Recombinant Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme is produced by our Mammalian expression system and the target gene encoding Leu18-Ser502 is expressed with a 6His tag at the C-terminus. 

Accession #: P05186

Known as: Alkaline Phosphatase; Tissue-Nonspecific Isozyme; AP-TNAP; TNSALP; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; ALPL

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP(PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia,characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY