Recombinant Human GNS/Glucosam

Recombinant Human GNS/Glucosamine (N-acetyl)-6-Sulfatase  (C354)

 

产品说明(Description)

Recombinant Human N-Acetylglucosamine-6-Sulfatase is produced by our Mammalian expression system and the target gene encoding Val37-Leu552 is expressed with a 6His tag at the C-terminus. 

Accession #: P15586

Known as: N-Acetylglucosamine-6-Sulfatase; Glucosamine-6-Sulfatase; G6S; GNS

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl,10% Glycerol,pH 8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

N-Acetylglucosamine-6-Sulfatase is a member of the Sulfatase family. N-Acetylglucosamine-6-Sulfatase is required for the lysosomal degradation of the Glycosaminoglycans (GAG) Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase hydrolyzes the 6-Sulfate groups of the N-Acetyl-D-Glucosamine 6-Sulfate units of Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase binds 1 Calcium ion per subunit. N-Acetylglucosamine-6-Sulfatase deficiency are the cause of Mucopolysaccharidosis Type 3D (MPS3D), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS3D has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

1、Two rheumatoid arthritis–specific autoantigens correlate microbial immunity with autoimmune responses in joints. Annalisa Pianta. et al. the journal of clinical investigation. 2017.

 

 

FOR RESEARCH USE ONLY