Recombinant Mouse Cathepsin H/

Recombinant Mouse Cathepsin H/CTSH  (CK47)

产品说明(Description)

Recombinant Mouse Cathepsin H is produced by our Mammalian expression system and the target gene encoding Glu22-Val333 is expressed with a 6His tag at the C-terminus. 

Accession #: AAA82966.1

Known as: Pro-cathepsin H; CTSH; ACC-4; ACC-5; aleurain; cathepsin B3; cathepsin BA; cathepsin H; CPSB; minichain; N-benzoylarginine-beta-naphthylamide hydrolase

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

 

质量控制(Quality Control) 

 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Cathepsin H (CTSH), which can act both as an aminopeptidase and as an endopeptidase, is a lysosomal cysteine protease of the papain family. CTSH is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. CTSH is associated with various pathological conditions like human fibrous meningioma, colorectal cancer, arthritis, human prostate tumor and lung cancer. CTSH is associated with cancer progression because of their ability to degrade extracellular matrices facilitating invasion, angiogenesis and metastasis as is evident from numerous clinical reports and experimental models. The expression of CTSH is significantly increased in disease states such as in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY