Recombinant Mouse BCMA/TNFRSF1

Recombinant Mouse BCMA/TNFRSF17  (CU46)

产品说明(Description)

Recombinant Mouse Tumor Necrosis Factor Receptor Superfamily Member 17 is produced by our Mammalian expression system and the target gene encoding Met1-Thr49 is expressed with a Fc tag at the C-terminus. 

Accession #: O88472

Known as: Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269; Tnfrsf17; Bcm; Bcma 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity-BLI:
Loaded Human APRIL-His(Cat#CU89) on HIS1K Biosensor, can bind  Mouse BCMA-Fc(Cat#CU46) with an affinity constant of 0.57 nM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

B cell maturation antigen (BCMA) is a member of the TNF receptor superfamily. It has been designated TNFRSF17. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development,function and regulation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY