- ￥240 - 600
- 近岸蛋白(Novoprotein)
- 中国
- C184
- 2026年01月30日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Recombinant Human UBE2D4/Ubiquitin-conjugating enzyme E2 D4
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ￥240.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥600.0 |
| 规格： | 500ug | 产品价格： | 询价 |
| 规格： | 1mg | 产品价格： | 询价 |
产品说明(Description)
Recombinant Human Ubiquitin-Conjugating Enzyme E2 D4 is produced by our E.coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus.
Accession #: Q9Y2X8
Known as: Ubiquitin-Conjugating Enzyme E2 D4; HBUCE1; Ubiquitin Carrier Protein D4; Ubiquitin-Protein Ligase D4; UBE2D4; UBCH5D
制剂（Formulation）
Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
质量控制（Quality Control）
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输（Shipping）
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存（Storage）
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景（Background）
Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification, protein K6-linked ubiquitination, protein K11-linked ubiquitination, protein K27-linked ubiquitination, protein K29-linked ubiquitination, protein K48-linked ubiquitination, and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
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文献和实验肝炎病毒包膜E2蛋白相同的亲和结合力3。另外，有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4） 利用Rho1D4纯化膜蛋白图解简述步骤1：结合：将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2：清洗：将杂蛋白和其他裂解液成分洗去，留下与亲和基质结合的目标蛋白。 步骤3：洗脱：过量Rho1D4肽与基质竞争性结合，释放出目标蛋白在洗脱液中收集。 表
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