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- 2026年03月29日
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- 文献和实验
- 技术资料
- 英文名：
Vacuum Trap Bottle 4l, UTP
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文献和实验In Vitro Transcription Assay (Run-off Assay) using Permeabil
for 5 min. 4. Cut a piece of nitrocellulose to the size of the slot-blot vacuum manifold. 5. Equilibrate the nitrocellulose membrane in 10X SSC. 6. Assemble the slot blot apparatus according to the manufacturer's instructions. 7. To the denatured DNA , add
InVitroTranscriptionAssay(Run-offAssay)usingPermeabil
for each slot of the slot blot 2. Incubate for 15 min at 85℃ to denature the DNA . 3. Incubate on ice for 5 min. 4. Cut a piece of nitrocellulose to the size of the slot-blot vacuum manifold. 5. Equilibrate the nitrocellulose membrane
Preparation of Nested Deletions
SSC made up to 800 ml in ddH2O), then place DNA side up on fresh 3MM Whatman or bench top paper to dry (1 hr). When all filters are dry, sandwich each between 3MM Whatman paper, and bake at 80℃ for 2 hrs under vacuum (need dry ice in trap). 10) Allow
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