pQE-30Xa Vector DNA

Preparing Vector DNA For Transfection/Targeting

  Since most people have their own preferences with this task you are completely at your leisure to ignore this section. This procedure works for me. However, be aware that highly purified DNA is important for high rates of targeting

Purification of Gene Targeting Vector DNA for Electroporation

1. Purify plasmid from bacteria. We recommend the Qiagen EndoFree Plasmid Maxi kit for the purification of the targeting vector plasmid from bacteria. Please follow the directions in the kit. Electroporation of Qiagen purified DNA has been used

载体 vector，cloning vector，carrier

　　载体 vector ， cloning vector ， carrier （ 1 ）指 DNA 重组实验使用的将被限制酶切断的供体 DNA 小片连起来令其繁殖的小型有自发繁殖能力的 DNA 分子。因用于 DNA 的克隆繁殖，所以也称克隆繁殖载体。一般是使用无传递性的小型质粒或病毒的 DNA ，但细胞器 DNA 或含有寄主染色体 DNA 的复制起始点的 DNA 小片也可用作载体。作为载体须具备下列条件：（ 1 ） DNA 可有效地注入活细胞中；（ 2 ）为了了解注入细胞