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Anti-FGF-2/bFGF Azide Free
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文献和实验Purification of Endotoxin Free mAbs
in endotoxin free water containing 10mM EDTA and subsequently stored sterile in endotoxin free water containing 0.1% sodium azide). Finally, centrifuge (at least 3500rpm for 30 min in bench centrifuge) and 0.2 micron sterile filter to remove any grossly
Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry
. Efficient copper-free azide-alkyne click reactions are, however, being developed, which should alleviate this constraint in the future.
Phycoerythrin conjugation protocol
. I used 117 x 1 cm. You can't really get away with anything smaller. Use a long thin one rather than a short fat one, since the latter did not work for me. Separation of the conjugate from the free reagents is tricky. *
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