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Anti-Derlin-1, clone 8G11.2
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文献和实验Clone Genes From a Phage Library
per ml as well). Both of these strains should be recovered from the freezer onto the appropriate drug plate and then kept in the refrigerator. Grow the bacteria in LB 0.2 percent maltose (to express the mal permease which is the lambda receptor) overnight
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
Purification Of Anti Peptide Antibody
peptide to the resin. Thaw serum- dilute 1:1 with TBS and filter through a 0.2 um filter Load the serum over the column, taking at least 20 minute total. Run the breakthrough over the column five times. Alternatively you can use
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