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ANTI-LKB1, CLONE 5C10 100UG
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文献和实验Single Clone Excision Protocol
tubes.Add 10 ml of phage supernatant to one,and 1 ml to the other (up to 100ml may be used if the excision hasn't gone well,but usually 1 ml is enough.) 10.Incubate tubes at 37℃ for 15 minutes. 11.Normally,the titer of transfected cells is so high
Clone Genes From a Phage Library
minutes to kill off the bacteria, then spin 15 min in the RC3 centrifuge at top speed. • Add 100, 10 and 1 microliters supernatant to 200 microliters SolR cells in 1.5 ml tube. Put to 37 degrees for 15 minutes. • Plate on LB amp
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
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