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文献和实验Plasmid Mini Purification Protocol
Important Notes Before Starting • New users are strongly recommended to read the QIAGEN handbook before starting the procedure. •Before using the kit for the first time, dissolve the lyophilized RNA se A provided Buffer PI . Buffer PI
Plant DNA methylation analysis by bisulfite genomic sequencing
and Wassenegger (2000). Back to top Procedure Preparation of Plant Genomic DNA Prepare plant genomic DNA using a Qiagen DNeasy Plant Maxi or Mini kit (see comment 1). Prior to the bisulfite-treatment, the plant genomic DNA is cleaved
Analysis of Proteins using Small Format 2D Gel Electrophoresis
the protocol with other virus-host cell systems. Between 2.2 x 10^7 and 7.1 x 10^8 TCID50 of virus are inoculated onto confluent HEp-2C cell monolayers prepared in 30mm petri dishes. After a 30 minute adsorption at room temperature the cells are overlaid
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