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文献和实验capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label a sample tube for each fluorochrome-conjugated rat or hamster antibody you are using, and add 1 drop of AbC™ RH capture beads
Intracellular Cytokine Staining Protocol
such as BSA or FCS can be added to the staining buffer. Optimal concentration of the fluorochrome-conjugated anti-cytokine antibodies has to be determined experimentally. To confirm specificity of the staining, it is common to block the directly-conjugated
在被轰击的皮肤区域产生灼伤效果。而如今的Naked DNA轰击技术可以很好地解决以上问题。相比于金粉包被DNA疫苗经常造成组织的轻微坏死与伤害,Naked DNA疫苗对机体组织的伤害较小。但之前的研究成果也有一部分原因可能是由于高压轰击造成。(Cheng WF, Hung CF, Lin KY, Ling M, Juang J, He L et al. CD8+ T cells, NK cells and IFN-gamma are important for control of tumor
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