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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
广州市左克生物
- CAS号:
129-17-9
- 规格:
10mM*1mL/100mg/500mg
| 规格: | 10mM*1mL | 产品价格: | ¥327.25 |
|---|---|---|---|
| 规格: | 100mg | 产品价格: | ¥297.5 |
| 规格: | 500mg | 产品价格: | ¥765.0 |
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文献和实验Partial proteolysis with Staph. aureus V8 protease
Materials: Overlay/Rehydration buffer: 125 mM Tris-HCl, pH 6.8 1mM EDTA 0.1% SDS 1mM 2-b-mercaptoethanol 30% glycerol some Bromophenol blue For 1 ml, you will need 0.35 ml of 2X buffer 0.30 ml H20
7.5. 3. 10% (w/v) NaN3 . 4. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.3. Fractionation of Wnt3A CM 2.3.1 Step 1: Blue Sepharose 1. Sample: 1–4 L Wnt3A CM as prepared in Section 2.2 . 2. Column: Blue Sepharose HP
Coomasie Blue Staining of Protein Gels
LEVEL I Materials Protein gel from Exercise 4.2 0.25% (w/v) Coomasie Brilliant Blue R 250 in methanol-water-glacial acetic acid (5-5-1), filtered immediately before use. 7% (v/v) acetic acid Commercial
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